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Blood was collected into 10-m L EDTA tubes (Becton, Dickinson and Company) for peripheral blood mononuclear cells (PBMCs) extraction, 2 EDTA tubes (4 m L each) to measure insulin, glucose, and alpha-1 acid glycoproteins levels, and 1 serum tube without clot activators (4 m L) to measure cytokine, MMP-9, lipid, and high-sensitivity C-reactive proteins concentrations. Blood samples collected in the EDTA tubes were kept in a chilled box with ice packs until processing.
JMIR Res Protoc 2025;14:e66417
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