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The control of Au concentrations follows the methodology described by Fiedler et al [19].
Au NPs with a diameter between 160 Å and 715 Å are prepared as described by Frens [20].
Fifty milliliters of 0.01% gold solution (by mass) should be transferred to the synthesis flask together with a magnetic bar and then placed in a previously heated oil bath. A watch glass should be placed on the mouth of the synthesis flask.
JMIR Res Protoc 2023;12:e49417
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